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Fracture damage of embryos and its prevention during vitrification and warming.

Author(s) : KASAL M., ZHU S. E., PEDRO P. B., NAKAMURA K., SAKURAI T., EDASHIGE K.

Type of article: Article

Summary

The frequency of fracture damage in mouse blastocyts was examined by repeated cycles of vitrification and warming. Fracture damage occurs during both cooling and warming, but it can be prevented completely by employing somewhat slower rates of cooling and warming. Furthermore, a high survival rate (88%) after 10 cycles of moderate cooling and moderate warming with 15 seconds of suspension in air indicates that vitrification, melting, or temperature fluctuation per se do not affect embryonic survival.

Details

  • Original title: Fracture damage of embryos and its prevention during vitrification and warming.
  • Record ID : 1997-1147
  • Languages: English
  • Source: Cryobiology - vol. 33 - n. 4
  • Publication date: 1996/08

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