High-pressure transient sensitization of Escherichia coli to lysozyme and nisin by disruption of outer-membrane permeability.

Author(s) : HAUBEN K. J. A., WUYTACK E. Y., SOONTJENS C. C. F., MICHIELS C. W.

Type of article: Article

Summary

Escherichia coli MG1655 suspensions in 10 milliM phosphate buffer (pH 7.0) were subjected to high pressures in the range of 180 to 320 megapascals for 15 minutes. Cell death was evident at 220 megapascals and increased exponentially with pressure. Surviving populations were sublethally injured. During exposure to high pressure (higher than 180 megapascals), cells were sensitive to lysozyme, nisin, and ethylenediaminetetraacetic acid (EDTA). Combinations of EDTA with nisin or lysozyme present during pressure treatment increased lethality in an additive manner.

Details

  • Original title: High-pressure transient sensitization of Escherichia coli to lysozyme and nisin by disruption of outer-membrane permeability.
  • Record ID : 1997-1501
  • Languages: English
  • Source: Journal of Food Protection - vol. 59 - n. 4
  • Publication date: 1996/04
  • Document available for consultation in the library of the IIR headquarters only.

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