Mammalian cell culture: essential techniques.

Author(s) : Wiley, DOYLE A., GRIFFITHS J. B.

Type of monograph: Book, Guide/Handbook


This book provides concise practical instructions for setting up a cell culture facility and operating it effectively and safely. Procedures are described to guide the readers from the initiation of primary cultures, handling established cell lines and quantifying their performance, through to the production of specialist cell types by imortalization. Contents include: introduction (laboratory facilities and tissue culture technique; safety aspects; methods available); basic ingredients for media; primary cultures; core techniques; core culture systems; quality assessment of cultures; immortalization; Suppliers details are given in the appendix to allow easy access to laboratory products.


  • Original title: Mammalian cell culture: essential techniques.
  • Record ID : 1999-0655
  • Languages: English
  • Publication: John wiley & sons - United kingdom/United kingdom
  • Publication date: 1997
  • ISBN: 0471970573
  • Source: Source: 174 p. (14 x 21.4); fig.; tabl.; append.; index; GBP 19.99.
  • Document available for consultation in the library of the IIR headquarters only.