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MULTIFACETED FREEZING INJURY IN HUMAN POLYMORPHONUCLEAR CELLS AT HIGH SUBFREEZING TEMPERATURES.

Author(s) : TAKAHASHI T., HAMMETT M. F., CHO M. S.

Type of article: Article

Summary

EXTRACELLULAR FREEZING INJURY AT HIGH SUBZERO TEMPERATURES IN HUMAN POLYMORPHONUCLEAR CELLS (PMNS) WAS STUDIED WITH A CRYOMICROSCOPE, ELECTRON MICROSCOPE, AND FUNCTIONAL ASSAYS (PHAGOCYTOSIS, MICROBICIDAL ACTIVITY, AND CHEMOTAXIS). FREEZING INJURY COMPRISES: OSMOTIC STRESS, CHILLING, COLD SHOCK, AND DILUTION SHOCK. EXTRACELLULARLY FROZEN PMNS LOSE FUNCTIONS WHEN COOLED TO 271 K (-2 DEG C) WITHOUT A CRYOPROTECTANT. GREATER DEHYDRATION PRODUCES IRREVERSIBLE INJURY TO CELLULAR FUNCTIONS, AND CELLS EVENTUALLY COLLAPSE UNDER HIGH OSMOTIC STRESS. CHILLING SENSITIVITY IS SEEN IN SLOWLY CHILLED, SUPERCOOLED PMNS BELOW 268 K (-5 DEG C) ; AT 266 K (-7 DEG C),FUNCTIONS ARE LOST IN 1 HR. THIS INJURY CAN BE PREVENTED BY THE ADDITION OF DMSO BUT NOT GLYCEROL. DMSO DOES NOT, HOWEVER, PREVENT COLD SHOCK INJURY (INJURY DUE TO RAPID COOLING), WHICH IS SEEN DURING COOLING AT 10 K/MIN TO 259 K (-14 DEG C), BUT NOT DURING SLOW COOLING AT 0.5 K/MIN. G.R.S.

Details

  • Original title: MULTIFACETED FREEZING INJURY IN HUMAN POLYMORPHONUCLEAR CELLS AT HIGH SUBFREEZING TEMPERATURES.
  • Record ID : 1986-0888
  • Languages: English
  • Source: Cryobiology - vol. 22 - n. 3
  • Publication date: 1985

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