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Response of epithelial (Madin-Darby canine kidney) cell junctions to calcium removal and osmotic stress is influenced by temperature.

Author(s) : ARMITAGE W. J., JUSS B. K., EASTY D. L.

Type of article: Article

Summary

Hypertonic stress caused a reduction in transepithelial electrical resistance both at 22 and 0 deg C, which, unlike the reduction caused by calcium removal at 22 deg C, was reversed relatively rapidly on return to isotonic conditions. Hypotonic stress increased transepithelial electrical resistance at 0 deg C, but had no effect at 22 deg C. The technique described here provides a straightforward method for investigating the effects of some of the stresses encountered during hypothermic storage and cryopreservation on intercellular junctions.

Details

  • Original title: Response of epithelial (Madin-Darby canine kidney) cell junctions to calcium removal and osmotic stress is influenced by temperature.
  • Record ID : 1995-1874
  • Languages: English
  • Source: Cryobiology - vol. 31 - n. 5
  • Publication date: 1994/10

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