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Coupled transport of water and cryoprotectant across cell membranes and applications to cryopreservation.

Number: pap. n. 003

Author(s) : WENG L., LI W.

Summary

The field of cryobiology has progressed as a fertile ground for new discoveries with important applications in cryopreservation over the past several decades. Some well-known thermodynamic models were proposed to predict the biophysical response of living cells and tissues in cryopreservation. This study, for the first time, develops a model that can calculate the coupled transport of water and cryoprotectants across cell membranes during the freezing and thawing protocols. The new model can eradicate the long-standing contradiction between the conventional Mazur model and the modern 2-P or K-K one. For cryobiological practice, the new model is applied to predict the volumetric change for human corneal keratocytes in the freezing protocol. The findings in this study will offer new insights into the biophysical response of biomaterials experiencing cryopreservation procedures.

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Format PDF

Pages: 246-251

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Details

  • Original title: Coupled transport of water and cryoprotectant across cell membranes and applications to cryopreservation.
  • Record ID : 30006655
  • Languages: English
  • Source: Cryogenics 2012. Proceedings of the 12th IIR International Conference: Dresden, Germany, September 11-14, 2012.
  • Publication date: 2012/09/11

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