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Large ice crystals in the nucleus of rapidly frozen liver cells.

Author(s) : BISCHOF J. C., RUBINSKY B.

Type of article: Article

Summary

Evidence in the literature shows that ice crystals that form in the nucleus of many rapidly cooled cells appear much larger than the ice crystals that form in the surrounding cytoplasm. The authors investigated the phenomenon in their laboratory using the techniques of freeze substitution and low temperature scanning electron microscopy on liver tissue frozen by liquid nitrogen plunge freezing. This method is estimated to cool the tissue at 1000 deg C/minute.

Details

  • Original title: Large ice crystals in the nucleus of rapidly frozen liver cells.
  • Record ID : 1994-2556
  • Languages: English
  • Source: Cryobiology - vol. 30 - n. 6
  • Publication date: 1993/12

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