ShareCryopreservation of in vitro shoot tips of chicory (Cichorium intybus L.).
Author(s) : DEMEULEMEESTER M. A. C., PANIS B. J., PROFT M. P. de
Type of article: Article
Summary
Cooling rates were varied between 0.1 and 0.7 deg C/min and the influence of DMSO (during preculture and cryoprotection treatments) on the survival was examined. The best result (regrowth of 83%) was obtained after 2 days preculture on medium without DMSO, cryoprotection with 15% DMSO and cooling to -40 deg C at a rate of 0.5 K/min prior to immersion in liquid nitrogen.
Details
- Original title: Cryopreservation of in vitro shoot tips of chicory (Cichorium intybus L.).
- Record ID : 1993-0267
- Languages: English
- Source: Cryo-Letters - vol. 13 - n. 3
- Publication date: 1992/05
- Document available for consultation in the library of the IIR headquarters only.
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Indexing
- Themes: Seeds and plants
- Keywords: Thawing; Cryoprotectant; Survival; Salad; Shoot; Vegetable; Chicory; Cryopreservation; Freezing
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Effect of various parameters on the survival of...
- Author(s) : HELLIOT B., BOUCAUD M. T. de
- Date : 1997/05
- Languages : English
- Source: Cryo-Letters - vol. 18 - n. 3
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Cryopreservation of cold-acclimated shoot tips ...
- Author(s) : SCOTTEZ C., CHEVREAU E., GODARD N., ARNAUD Y., DURON M., DEREUDDRE J.
- Date : 1992/12
- Languages : English
- Source: Cryobiology - vol. 29 - n. 6
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Cryopreservation of alginate-coated in vitro gr...
- Author(s) : VANDENBUSSCHE B., DEMEULEMEESTER M. A. C., PROFT M. P. de
- Date : 1993/09
- Languages : English
- Source: Cryo-Letters - vol. 14 - n. 5
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INFLUENCE OF DAYS OF CULTURE ON CRYOPROTECTANT-...
- Author(s) : MCADAMS S., RATNASABAPATHI D., SMITH R. A.
- Date : 1991/06
- Languages : English
- Source: Cryobiology - vol. 28 - n. 3
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SURVIVAL AT ULTRA-LOW TEMPERATURES OF SHOOT TIP...
- Date : 1984
- Languages : English
- Source: Cryo-Letters - vol. 5 - n. 5
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