Share

Use of confocal laser scanning microscope in conjunction with a conduction heat transfer stage in order to observe dynamically the freeze-thaw cycle in an autofluorescent substance and to measure ice crystal size in situ.

Author(s) : EVANS J., ADLER J., MITCHELL J., BLANSHARD J., RODGER G.

Type of article: Article

Summary

The method involves the use of a heat transfer stage on a confocal laser scanning microscope, for which there is no need for very thin slices to be cut. It does require the sample to fluoresce, either by being autofluorescent or by having absorbed sufficient fluorophore. The technique is applied to mycoprotein, an autofluorescent food material, under the conditions employed, the structure induced on freezing is lost on thawing. It is also applied to fluorescently labeled egg albumin gel; in this case the structure induced on freezing is permanent immediately. Ice crystal size determination is carried out for water (containing fluorescein), egg albumin solution, egg albumin gel, heat-treated suspension of Fusarium graminearum, and mycoprotein.

Details

  • Original title: Use of confocal laser scanning microscope in conjunction with a conduction heat transfer stage in order to observe dynamically the freeze-thaw cycle in an autofluorescent substance and to measure ice crystal size in situ.
  • Record ID : 1997-0305
  • Languages: English
  • Source: Cryobiology - vol. 33 - n. 1
  • Publication date: 1996/02

Links


See other articles in this issue (11)
See the source

Indexing